The following highways are numbered 143:

Canada

• Prince Edward Island Route 143
• Quebec Route 143

Costa Rica

• National Route 143

Germany

• Bundesautobahn 143

Japan

• Japan National Route 143
• Fukuoka Prefectural Route 143
• Nara Prefectural Route 143

Malaysia

• Malaysia Federal Route 143

United States

• U.S. Highway 143 (former)
• Alabama State Route 143
• Arizona State Route 143
• Arkansas Highway 143
• California State Route 143 (unbuilt)
• Florida State Road 143
  • County Road 143 (Hamilton County, Florida)
• Georgia State Route 143 (former)
• Illinois Route 143
• Indiana State Road 143
• Iowa Highway 143
• K-143 (Kansas highway)
• Kentucky Route 143
• Louisiana Highway 143
• Maine State Route 143
• Maryland Route 143 (former)
• Massachusetts Route 143
• M-143 (Michigan highway) (one former and one current highway)
• Missouri Route 143
• New Jersey Route 143
• New Mexico State Road 143
• New York State Route 143
  • County Route 143 (Fulton County, New York)
  • County Route 143 (Niagara County, New York)
  • County Route 143 (Wayne County, New York)
  • County Route 143 (Westchester County, New York)
• North Carolina Highway 143
• Ohio State Route 143
• Oklahoma State Highway 143 (former)
• Pennsylvania Route 143
• Tennessee State Route 143
• Texas State Highway 143 (former)
  • Texas State Highway Loop 143
  • Texas State Highway Spur 143 (former)
  • Farm to Market Road 143
• Utah State Route 143
• Vermont Route 143
• Virginia State Route 143
  • Virginia State Route 143 (1923-1928) (former)
  • Virginia State Route 143 (1933-1942) (former)
• Washington State Route 143 (two former highways)
• Wisconsin Highway 143

Territories

• Puerto Rico Highway 143