Mary Osborn or Osborne may refer to:
- Mary Osborn (born 1940), English cell biologist
- Mary Jane Osborn (1927–2019), American biochemist and molecular biologist
- Mary Osborne (1921–1992), American jazz guitarist and guitar manufacturer
- Mary Pope Osborne (born 1949), American author
- Mary Osborne, Duchess of Leeds (1723–1764), noblewoman
See also
- Mary Osbourne (born 1982), winner of the MTV reality television show Surf Girls
- Emily Mary Osborn (1828–1925), English painter of the Victorian era
- Mary Osburn Adkinson (born Mary Almira Osburn, 1843–1918), American social reformer active in the temperance movement
- Mary Osborn Douthit (1850–1908), early white Oregon settler, teacher, and advocate of woman suffrage
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