Janoušek (feminine Janoušková) is a Czech surname, it may refer to the following notable people:
- Anna Janoušková, Czech cross-country skier
 - Antonín Janoušek, Czech communist
 - Bohumil Janoušek, Czech rower
 - Gabriel Janoušek, Czech canoer
 - Jiří Janoušek, Czech footballer
 - Karel Janoušek, Czechoslovak Air Force commander
 - Ladislav Janoušek, Czech cyclist
 - Miloš Janoušek (1952–2023), Slovak folk musician
 - Roman Janoušek, Czech lobbyist
 - Roman Janoušek, Czech footballer
 - Václav Janoušek, Czech canoer
 - Věra Janoušková, Czech sculptor, painter and graphic artist
 
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